Uricase from Arthrobacter globiformis

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  Selectoprobe™ Horseradish Peroxidase (HRP) (High Purity, ≥250U/mg)

  Catalog No.: SP-F-0159

  Description: Selectoprobe™ Horseradish Peroxidase (HRP) is a high-purity horseradish peroxidase enzyme designed for optimal use in activity assays and antibody conjugation applications, including ELISA, Western blot, and immunohistochemistry.

  Key Features of Selectoprobe™ Horseradish Peroxidase:
  - Purified and Ready-to-Use: Lyophilized, salt-free powder form for easy dissolution and immediate use.
  - High Specific Activity: Typically greater than 300 units/mg (with lot-specific activity values provided).
  - Superior to AP: HRP (40kDa) is smaller and has a higher specific enzyme activity compared to alkaline phosphatase (AP; 140kDa) and beta-galactosidase (b-Gal).
  - Versatile Applications: Compatible with numerous substrate solutions and assay techniques, providing flexibility in assay design.

  Supplied as a lyophilized, salt-free powder, this HRP preparation is ideal for molecular biology and protein research applications, particularly as a reporter system in immunoassays and probe-based methods like ELISA, Western blotting, EMSA, and Southern blotting. Typically conjugated to secondary antibodies or streptavidin, HRP activity can be visualized using colorimetric or luminescent substrates.

  Horseradish peroxidase (HRP) interacts with hydrogen peroxide (H₂O₂) to form an [HRP-H₂O₂] complex, which can oxidize a broad range of hydrogen donors, including various substrates used in detection. These substrates include colorless, reduced chromogenic substrates (e.g., DAB, TMB, ABTS), chemiluminescent substrates (e.g., luminol), and fluorescent substrates (e.g., Amplex Red). Upon incubation with these substrates, HRP produces a colored, luminescent, or fluorescent product, allowing quantification through colorimetric, luminescent, or fluorimetric detection methods.

  HRP can be conjugated to antibodies using several techniques, such as glutaraldehyde, periodate oxidation, disulfide linkage, or amino- and thiol-directed cross-linkers. Compared to β-galactosidase and alkaline phosphatase, HRP’s smaller size and enhanced stability make it a preferred labeling enzyme for many applications.

  Storage: -20 °C; Minimize light exposure

   

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  Selectoprobe™ polyHRP-Goat Anti-Rabbit IgG Antibody Conjugate

  Catalog No.: SP-F-0157

  Description: Goat anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.

  Selectoprobe™ polyHRP-Goat Anti-Rabbit IgG Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The goat anti-rabbit IgG poly-HRP conjugate is purified to remove unconjugated goat anti-mouse IgG molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. Selectoprobe™ polyHRP-Goat Anti-Rabbit IgG is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

  Storage: Refrigerated (2-8 °C); Minimize light exposure

   

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  Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis(hexafluorophosphate)

  Catalog No: SP-O-0002
  Purity: 95%-98%
  CAS No: 136724-73-7
  Synonym: Ru(bpy)₂(mcbpy-O-Su-ester)(PF₆)₂
  MW: 1014.66
  Formula: C₃₆H₂₉F₁₂N₇O₄P₂Ru

   

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  Ruthenium bis(bipyridine)dicarboxybipyridine

  Catalog No: SP-O-0001
  Purity: 95%-98%
  CAS No: 97333-46-5
  Synonym: Ruthenate(4-),tris[[2,2'-bipyridine]-4,4'-dicarboxylato(2-)-N1,N1']-,hydrogenchloride(1:6:2)
  MW: 904.58
  Formula: C₃₆H₂₄Cl₂N₆O₁₂Ru

   

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