Hydrogen ionophore IV (ETH 1778) — High Purity for ISE | Selectoprobe

References

Oesch U, Brzózka Z, Xu A, et al. Solvent polymeric membrane pH catheter electrode for intraluminal measurements in the upper gastrointestinal tract . Med. Biol. Eng. Comput, 1987, 25:414-419; DOI: 10.1007/BF02443362.

References

Kong, Hui et al. A RT-LAMP based hydrogen ion selective electrode sensing for effective detection HIV-1 RNA with high-sensitivity . Sensors and Actuators B-chemical, 2020, 329: 129118.

Abstract

Development of a simple, sensitive and specific method for HIV-1 assay is demanded. In some emergency situations, a portable reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is necessary. A novel hydrogen ion-selective RT-LAMP (H⁺-RT-LAMP) sensor was developed for highly sensitive HIV-1 using hydrogen ion concentration as a signal producing compound. The H⁺-RT-LAMP sensor was based on screen-printed with carbon-doped graphene conductive layer, carbon nanotubes (CNTs) solid electrolyte layer, and reference electrode KCl functional layer, which provided superior electrical signals, high sensitivity and stability for HIV-1 detection. The rate of hydrogen ion production was related to the viral load (VL) during the amplification of HIV-1. The sensitive HIV-1 RNA detection range was achieved from 0 copies/μL to 10⁵ copies/μL. The proposed H⁺-RT-LAMP sensor was applied to detect HIV-1 RNA in real human serum. 57 samples were detected and satisfactory results were obtained. Compared with commercial instruments, the detection results of H⁺-RT-LAMP sensor demonstrated a perfect concordance rate of 96.5 %, and the limit of detection (LOD) for the clinical samples was 10 copies per tube. Time to get the result is from 10 min to 25 min. Therefore, the proposed H⁺-RT-LAMP sensor was particularly suitable as a point-of-care device for the on-site detection of HIV-1.

Octadecyl isonicotinate | C24H41NO2 | CID 517813 - PubChem (nih.gov)

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