DBCO-NHCO-PEG5-NHS ester

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  Description: This lyophilized Selectoprobe™ Eu-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Selectoprobe™ Eu covalently attached (as the time-resolved red fluorescent europium label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using TR-FRET platform. A variety of the complementary biotinylated reagents are available from Selectoprobe™.

  Storage: Freeze (< -15 °C); Minimize light exposure

  Correction Factor (260 nm)	0.911
  Correction Factor (280 nm)	0.777
  Extinction coefficient (cm -1 M -1)	21000
  Excitation (nm)	298
  Emission (nm)	617

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  Selectoprobe™ polyHRP-Goat Anti-Mouse IgG Antibody Conjugate

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  Description: Goat anti-mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.

  Selectoprobe™ polyHRP-Goat Anti-Mouse IgG Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The goat anti-mouse IgG poly-HRP conjugate is purified to remove unconjugated goat anti-mouse IgG molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. Selectoprobe™ polyHRP-Goat Anti-Mouse IgG is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

  Storage: Refrigerated (2-8 °C); Minimize light exposure

   

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  Clickable chromoionophore I

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  Purity: 95%-98%
  CAS No: N/A
  Synonym: (Z)-N-(9-(diethylamino)-5H-benzo[a]phenoxazin-5-ylidene)hept-6-ynamide
  MW: 425.53
  Formula: C₂₇H₂₇N₃O₂

   

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  Selectoprobe™ Europium Cryptate HTRF Labeling Reagent (Eu cryptate DBCO)

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  Description: The Europium cryptate labeling reagent is specifically designed for biomolecule labeling, enabling the rapid and efficient development of time-resolved fluorescence resonance energy transfer (TR-FRET) assays. It facilitates quick and easy labeling of peptides, proteins, and oligonucleotides.

  The Selectoprobe™ Eu DBCO (europium complex) can be readily clicked to molecules containing an azido group under copper-free conditions.

  Compared to the other compounds, our cryptate Eu probes have extremely high stability, high emission yield and ability to selectively label biomolecules. Moreover, our Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.

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