N,N'-bis(salicylidene)ethylenediaminatooxovanadium(IV)
$240.00 – $546.00
Catalog No: Custom 007
CAS No: 36913-44-7
MW: 333.24
Catalog No | Custom 007 |
---|---|
CAS No | 36913-44-7 |
Weight | 50mg, 100mg, 200mg |
Lead Time | 2-3 days |
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Selectoprobe™ Horseradish Peroxidase (HRP) (High Purity, ≥250U/mg)
Catalog No.: SP-F-0159
Description: Selectoprobeâ„¢ Horseradish Peroxidase (HRP) is a high-purity horseradish peroxidase enzyme designed for optimal use in activity assays and antibody conjugation applications, including ELISA, Western blot, and immunohistochemistry.
Key Features of Selectoprobeâ„¢ Horseradish Peroxidase:
- Purified and Ready-to-Use: Lyophilized, salt-free powder form for easy dissolution and immediate use.
- High Specific Activity: Typically greater than 300 units/mg (with lot-specific activity values provided).
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- Versatile Applications: Compatible with numerous substrate solutions and assay techniques, providing flexibility in assay design.Supplied as a lyophilized, salt-free powder, this HRP preparation is ideal for molecular biology and protein research applications, particularly as a reporter system in immunoassays and probe-based methods like ELISA, Western blotting, EMSA, and Southern blotting. Typically conjugated to secondary antibodies or streptavidin, HRP activity can be visualized using colorimetric or luminescent substrates.
Horseradish peroxidase (HRP) interacts with hydrogen peroxide (Hâ‚‚Oâ‚‚) to form an [HRP-Hâ‚‚Oâ‚‚] complex, which can oxidize a broad range of hydrogen donors, including various substrates used in detection. These substrates include colorless, reduced chromogenic substrates (e.g., DAB, TMB, ABTS), chemiluminescent substrates (e.g., luminol), and fluorescent substrates (e.g., Amplex Red). Upon incubation with these substrates, HRP produces a colored, luminescent, or fluorescent product, allowing quantification through colorimetric, luminescent, or fluorimetric detection methods.
HRP can be conjugated to antibodies using several techniques, such as glutaraldehyde, periodate oxidation, disulfide linkage, or amino- and thiol-directed cross-linkers. Compared to β-galactosidase and alkaline phosphatase, HRP’s smaller size and enhanced stability make it a preferred labeling enzyme for many applications.
Storage: -20 °C; Minimize light exposure
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Selectoprobeâ„¢ Eu goat anti-rabbit IgG (H+L) (Cross Adsorbed Antibody)
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Description: The Selectoprobeâ„¢ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobeâ„¢ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. This lyophilized Selectoprobeâ„¢ Eu goat anti-rabbit IgG (H+L) conjugate (Cross Adsorbed Antibody) is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.
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Correction Factor (260 nm)
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Correction Factor (280 nm)
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Extinction coefficient (cm -1 M -1)
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Excitation (nm)
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Emission (nm)
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Selectoprobeâ„¢ Eu donkey anti-goat IgG (H+L) (Cross Adsorbed)
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